16SrRNA: 2015 LYCOG soils - SRA

SRX3413547: 16SrRNA: 2015 LYCOG soils
1 ILLUMINA (Illumina MiSeq) run: 238,266 spots, 143.4M bases, 105.5Mb downloads

Design: Extract DNA; Flourometric quantification(FQ);First step PCR with 515f and 926r primers with adapters and second step PCR with Illumina adapters; Spike libraries 20% phiX;MiSeq sequence with Reagent Kit v3 2X300;combine paired ends with join-fastq.

Submitted by: Baylor University

Study: Soil samples Lysimeter CO2 gradient facility USDA, Temple, TX

PRJNA416942 • SRP125406 • All experiments • All runs

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The objective of this study was to characterize soil microbial communities under a decade long preindustrial-to-future CO2 gradient in three Texas Prairie soils

Sample: 16SrRNA: 2015 soils

SAMN08046110 • SRS2706679 • All experiments • All runs

Organism: soil metagenome

Library:

Name: B55e1

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: METAGENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 238,266 spots, 143.4M bases, 105.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR6313561	238,266	143.4M	105.5Mb	2017-11-21

ID:: 4757630

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